제주도에서 분리한 미생물의 수질정화 성능 평가에 관한 연구

초록

In this study, we collected samples from rivers in Jeju Island and other places where the self-purification capacity of rivers had been recovered by utilizing effective microorganisms(EM). And then after separating microorganisms from among the samples, we evaluated each microorganism’s water purification. In addition, we performed DNA sequencing by means of the 16S rRNA gene (16S rDNA) sequencing method for the identification of microorganisms capable of improving water quality and sought to register gene sequence information in GenBank at the National Center for Biotechnology Information (NCBI). According to the COD experiment results, C1, B4 and C6 showed the average removal efficiency of 21.3, 23.4 and 38.6% respectively. According to the T-N experiment results, the respective strains of B4, B6, M3 and C1 showed the average removal efficiency of 26.8, 34.9, 28.3 and 23.3% respectively. According to the T-P experiment results, the respective strains of B4, B6, C1 and C4 showed the average removal efficiency of 15.0, 19.4, 15.1 and 9.1% respectively. By examining the present study, it is judged that the strain of B6 has the best water purification performance. As a result of its identification, the strain of B6 showed 99.66% similarity to the strain of Bacillus subtilis. Moreover, the gene sequence information was registered in GenBank at NCBI in the USA, thereby being assigned the accession number of ‘KU672511’.

키워드